Abstract
A novel fluorescent probe (Flu-Si) based on fluorescein and coumarin fluorophores for highly selective and sensitive detection of F− was successfully developed. It was shown that the mechanism of recognition involves a desilylation reaction that takes place after addition of F− anions. A linear relationship between the ratio of emission intensities at 532 and 465 nm (I532/I465) and F− concentration over the range of 0–20 μmol L−1 with a LOD of 0.025 μmol L−1 was found. The sensing mechanism of Flu-Si with F− was confirmed by density functional theory (DFT) and time dependent density functional theory (TDDFT) calculation. Moreover, it was demonstrated that Flu-Si is an efficient fluorescent probe for F− anions in living cells and zebrafish, indicating its sensing potential in clinical diagnosis.
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