Abstract
AbstractA biosensor for phosphate determination with the flow‐injection system was developed using rhodium nanoparticles modified Poly(pyrrole‐co‐[1‐(2‐aminophenyl) pyrrole])/pyruvate oxidase. The biosensor showed a very wide linearity up to 70 mM phosphate concentration compared to previously reports, response time of 4 s., operational stability with a relative standard deviation of 0.009 % and accuracy of 99.4 %±0.949 at a flow rate of 2.0 Ml min.−1 at exactly −0.68 V. Detection limit were calculated to be 21±0.001 μM by preserving 81.1 % of its initial response at the end of 16th days. Artificial urine was analyzed without dilution to investigate biosensor performance.
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