Abstract

BackgroundGastric cancer (GC) is a common malignant cancer with a poor prognosis. Ferroptosis has been shown to play crucial roles in GC development. Long non-coding RNAs (lncRNAs) is also associated with tumor progression in GC. This study aimed to screen the prognostic ferroptosis-related lncRNAs and to construct a prognostic risk model for GC.MethodsFerroptosis-related lncRNAs from The Cancer Genome Atlas (TCGA) GC expression data was downloaded. First, single factor Cox proportional hazard regression analysis was used to select seven prognostic ferroptosis-related lncRNAs from TCGA database. And then, the selected lncRNAs were further included in the multivariate Cox proportional hazard regression analysis to establish the prognostic model. A nomogram was constructed to predict individual survival probability. Finally, we performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) to verify the risk model.ResultsWe constructed a prognostic ferroptosis-related lncRNA signature in this study. Kaplan-Meier curve analysis revealed a significantly better prognosis for the low-risk group than for the high-risk group (P = 2.036e-05). Multivariate Cox proportional risk regression analysis demonstrated that risk score was an independent prognostic factor [hazard ratio (HR) = 1.798, 95% confidence interval (CI) =1.410–2.291, P < 0.001]. A nomogram, receiver operating characteristic curve, and principal component analysis were used to predict individual prognosis. Finally, the expression levels of AP003392.1, AC245041.2, AP001271.1, and BOLA3-AS1 in GC cell lines and normal cell lines were tested by qRT-PCR.ConclusionsThis risk model was shown to be a novel method for predicting prognosis for GC patients.

Highlights

  • Gastric cancer (GC) is a common malignant cancer with a poor prognosis

  • This study showed that the prognostic ferroptosisrelated Long non-coding RNAs (lncRNAs) signature was a reliable prognostic predictor for GC

  • We used the data of 407 GC patients from The Cancer Genome Atlas (TCGA) cohort (T = 375, N = 32)

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Summary

Introduction

Gastric cancer (GC) is a common malignant cancer with a poor prognosis. Ferroptosis has been shown to play crucial roles in GC development. Long non-coding RNAs (lncRNAs) is associated with tumor progression in GC. This study aimed to screen the prognostic ferroptosis-related lncRNAs and to construct a prognostic risk model for GC. Gastric cancer (GC) is a common cancer. It was the third leading cause of cancer-related deaths in 2018 [1]. The incidence and mortality rates of GC have increased in China [2]. Ferroptosis is a non-apoptotic form of cell death [4]. Ferroptosis is associated with small molecules that target the antioxidant system or enzymes such as system xc − and glutathione peroxidase 4 (GPX4) [5].

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