Abstract
Exopolysaccharides produced by Weissella cibaria has attracted increasing attention owing to their biological activity. Here, a strain was isolated from the home-made fermented octopus, which was identified as W. cibaria FAFU821. In addition, the polysaccharide were isolated and purified by cellulose DE-52 column and Sephadex G-100 column, and named EPS821-1. In this work, the structure of EPS821-1 and its cryoprotective activity on Bifidobacterium longum subsp. longum F2 were investigated in vitro. These results suggested that the EPS821-1 is a novel glucan, which mainly consists of α-(1 → 6) linkage with α-(1 → 4), α-(1 → 4,6) and α-(1 → 3,6) residue as branches. In addition, EPS821-1 existed the three-dimensional network structure and exhibited the excellent cryoprotective activities for B. longum subsp. longum F2, which was 2.75 folds higher than that of the controls. This study provided scientific evidence and insights for the application of EPS821-1 as cryoprotection in food field.
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