Abstract
In this research, a new sequential batch reactor (SBR) coupled to an internal magnetic separator was developed. The separator consists of a set of axially magnetized permanent toroidal magnets, distributed along a non-magnetic steel rod, uniformly spaced with alternate polarity, which provide an external magnetic field of up to 1.2 T. The feasibility of magnetic separation was assessed for the retention of the nanobiocatalyst based on laccase immobilized on silica-coated MNPs. The proof of concept was evaluated for the enzymatic decolorization of the methyl green (MG) dye and the oligomerization of rutin, with virtually complete recovery of the nanobiocatalyst (99%) and high biotransformation efficiency of both compunds. The characterization of the reaction products of MG by laccase was conducted by high performance liquid chromatography (HPLC) with a diode array detector (DAD) coupled to mass spectrometry (MS) using the electrospray ionization source (ESI) (HPLC-DAD-ESI-MS). Moreover, the biotransformation products showed less microtoxicity than the parent compound and increased biodegradability. Beyond laboratory scale experiments, the reactor proposed here was scaled-up to a volume of 100 L and the environmental performance and cost analysis were estimated.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
