A novel enzymatic biosensor for detection of intracellular hydrogen peroxide based on 1-aminopyrene and reduced graphene oxides

Abstract

An electrochemical sensor for hydrogen peroxide $$(\text {H}_{2}\text {O}_{2})$$ detection was established using 1-aminopyrene (AP) as a linker between horseradish peroxidase (HRP) and reduced graphene oxides (rGO) modified glassy carbon electrode. The AP can combine with HRP via a covalent bond and combine with rGO via $$\uppi $$ - $$\uppi $$ conjugation. The covalent bond linkages can prevent the enzymes leaching of the enzymes and the $$\uppi $$ - $$\uppi $$ conjugate combination can facilitate electrons transfer, which plays synergistic effects to improve the performance of this sensor. The electrochemical activity of the HRP-AP/rGO was described by cyclic voltammetry, alternating current impedance and amperometric techniques. Under the optimal conditions, the biosensor showed a wide linear range from 1.5 $$\upmu $$ M to 28.5 $$\upmu $$ M and a low detection limit of 0.5 $$\upmu $$ M with good stability and high selectivity, confirming that the sensor is well-suited for the detection of $$\text {H}_{2}\text {O}_{2}$$ during biological processes. Using 1-aminopyrene (AP) as a linker between horseradish peroxidase (HRP) and reduced graphene oxides (rGO), an enzymatic hydrogen peroxide ( $$\text {H}_{2}\text {O}_{2})$$ biosensor was established with wide linear range, good stability and high selectivity.