Abstract
We have developed an ELISA procedure for the detection of C3 nephritic factor (C3NeF), in which wells are coated with a fixed concentration of 2 μg C3b per well, and subsequently reacted with B and D. The presence of increasing concentrations of NiCl 2 showed a NiCl 2 concentration-dependent generation of C3bBb and very little solid-phase bound C3bBb was generated with MgCl 2. The formation of solid-phase C3bBb in the presence of an optimal concentration of 1 mM NiCl 2, was time-dependent and plateau values were reached after 30 min at 37°C. IgG purified from the serum of a patient with membranoproliferative glomerulonephritis (MPGN) type II containing C3NeF stabilizing activity was bound to the C3bBb generated on microwells in a dose-dependent manner whereas normal IgG exhibited only minor reactivity. C3NeF activity was measured using the ELISA method in patients with MPGN type II ( n = 15) and other diseases ( n = 17) and in normal controls ( n = 15). Most of the patients with MPGN type II exhibited positive C3NeF at various levels, while two of the disease controls showed only slight reactivities. C3NeF titers measured by this new ELISA procedure correlated well with previously described hemolytic assays ( r = 0.617, p < 0.01).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
