Abstract
In this work, a novel electrochemiluminescence (ECL) biosensing system for the sensitive and selective detection of choline was creatively designed based on the smart integration of multifunctional nanocomposites and a certain enzymatic reaction. Firstly, graphene-Au-chitosan (GR-Au-CS) nanocomposite, which possesses the property of intensification effect on luminol ECL, was electrochemically deposited on the bare GCE. Then, a biofunctional Fe3O4-TiO2-ChO biocomposite was unprecedentedly synthesized with combining the mimic peroxidase activity of Fe3O4 and the intensification effect of TiO2 on the luminol ECL. Thus, on the basis of smart integration of the above nanocomposites and the detection of H2O2 generated by ChO enzymatic reactions under the optimal conditions, the obtained ECL biosensor for choline showed wide linear range from 3nM to 1.12mM with detection limit (signal-to-noise ratio of 3) of 1nM. The excellent performance of the biosensor shows that this method opens up a new avenue in the practical application.
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