Abstract
A novel amperometric immunosensor was fabricated using an electropolymerized conducting polymer composite of pyrrole and 4-(3-pyrrolyl) butyric acid as the probe immobilization matrix. The biomolecule was immobilized via covalent bonding on the polymer surface with a high density of carboxyl groups. Instead of directly attaching the probe to the polymer surface, the streptavidin-biotin affinity complex was introduced to improve the bioactivity, orientation, and the amount of immobilized material. Polyvinyl alcohol was employed to improve the hydrophilicity of the polymer surface and further decrease the nonspecific protein adsorption. As a result, high sensitivity and specificity were achieved with a wide linear dynamic range. Using mouse immunoglobulin G as a model protein, the immunosensor exhibited a linear relationship between 7 ng/mL and 20 µg/mL with a limit of detection of 7 ng/mL.
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