Abstract
Foods contaminated by foodborne pathogens have always been a great threat to human life. Herein, we constructed an electrochemical immunosensor for Salmonella detection by using a Fe3O4@graphene modified electrode. Because of the excellent electrical conductivity and mechanical stability of graphene and the large specific surface area of Fe3O4, the Fe3O4@graphene nanocomposite exhibits an excellent electrical signal, which greatly increased the sensitivity of the immunosensor. Gold nanoparticles were deposited on Fe3O4@graphene nanocomposite by electrochemical technology for the immobilization of the antibody. Cyclic voltammetry was selected to electrochemically characterize the construction process of immunosensors. The microstructure and morphology of related nanocomposites were analyzed by scanning electron microscopy. Under optimized experimental conditions, a good linear relationship was achieved in the Salmonella concentration range of 2.4 × 102 to 2.4 × 107 cfu/mL, and the limit of detection of the immunosensor was 2.4 × 102 cfu/mL. Additionally, the constructed immunosensor exhibited acceptable selectivity, reproducibility, and stability and provides a new reference for detecting pathogenic bacteria in milk.
Highlights
Foods contaminated by foodborne pathogens have caused by Salmonella every year (Zhu et al, 2014)
Electrochemical Procedures All electrochemical measurements were tested in PBS containing [Fe(CN)6]3−/4− (5 mM) as probe
Further magnification showed that a very rough and rugged surface can be clearly observed (Figure 2c). Benefiting from this structure and shape, the surface area of Fe3O4 was greatly increased, and it provides abundant sites for the subsequent deposition of AuNPs, which plays a unique role in reducing the detection limit of the sensor (Gao et al, 2013)
Summary
Foods contaminated by foodborne pathogens have caused by Salmonella every year (Zhu et al, 2014). We present, more than 2,500 serotypes of Salmonella have constructed an electrochemical immunosensor for Sal- been identified (Gast, 2007). Because of the excellent electrical conductiv- mon cause of food poisoning in humans and animals ity and mechanical stability of graphene and the large (Hartmann and West, 1995; Hassan et al, 2017). Specific surface area of Fe3O4, the Fe3O4@graphene Normally, after eating Salmonella contaminated food, nanocomposite exhibits an excellent electrical signal, humans, especially children, may experience symptoms which greatly increased the sensitivity of the immuno- such as vomiting, headache, and diarrhea Metry was selected to electrochemically characterize raw milk is contaminated by Salmothe construction process of immunosensors. Foodborne pathogens are mainly divided into 2 categories; one is traditional culture plating, and the other is instrumental analysis technology (Ye et al, 2019)
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