Abstract
Listeria monocytogenes (L. monocytogenes), as one of the most common foodborne pathogens, has caused many foodborne diseases, posing a major threat to food and public health safety. Therefore, a novel electrochemical biosensor was proposed based on the combination of saltatory rolling circle amplification (SRCA) and nicking enzyme mediated amplification (NEMA) for detecting Listeria monocytogenes. When L. monocytogenes was present, abundant double-stranded DNA (dsDNA) were produced by SRCA reaction. SRCA products could be specifically recognized and cleaved by Nb.BsmI. SRCA-NEMA was performed to generate abundant single-stranded DNA (ssDNA) with the action of Bst DNA polymerase and Nb.BsmI. The hydrosulfuryl-modified hairpin DNA (HS-hpDNA) was immobilized on the electrode surface and G-quadruplex was used as signaling molecule. The current signal increased with target DNA concentration and the ratio of the current could be calculated as the result. Subsequently, the detection linear range for L. monocytogenes genomic DNA was 5.4 × 100-5.4 × 107 fg/μL, with the detection limit of 2.13 fg/μL. In comparison with ISO 11290-1:2017 method, the relative sensitivity, relative specificity and relative accuracy of the developed method were 100 %, 97.8 % and 98 %, respectively. Overall, the developed method provides an outstanding platform for sensitive detection of L. monocytogenes in food.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
