A novel electrochemical biosensor based on dual signal amplification of CMK-3@AuNPs and ATRP for DR1 detection

Abstract

Down-regulator of transcription 1 (DR1) is a crucial biomarker closely related to Hashimoto's Thyroiditis (HT). In this study, a highly selective electrochemical biosensor for ultrasensitive detection of DR1 was prepared by combining nanomaterials CMK-3 @AuNPs and atom transfer radical polymerization (ATRP) signal amplification strategy for the first time. Based on the specific recognition of antigen and antibody, the sensor has strong selectivity. CMK-3 @AuNPs can not only increase the conductivity of the electrode, but also provide more active sites for biological molecules, so as to effectively amplify the output signal. The FMMA monomers were polymerized on the electrode surface through ATRP reaction, which made the electrochemical signal increased significantly. Under optimal conditions, the fabricated electrochemical biosensor showed a wide detection range of 5 × 10-4 ng/mL ∼ 5 × 102 ng/mL with a detection limit as low as 0.159 pg/mL. In addition, the electrochemical biosensor has good stability and anti-interference ability. Therefore, the sensor has broad prospects in the detection of biomarkers.