A novel electro analytical nanosensor based on graphene oxide/silver nanoparticles for simultaneous determination of quercetin and morin

Abstract

In this report, silver nanoparticles (AgNPs) with the mean diameters of 10-30nm were self-assembled onto the surfaces of 2-aminoethanethiol (2-AET) functionalized graphene oxide (AETGO) sheets. The graphene oxide (GO) and AgNPs-AETGO nanocomposites were characterized by a transmission electron microscope (TEM), x-ray photoelectron spectroscopy (XPS), reflection–absorption infrared spectroscopy (RAIRS) and the x-ray diffraction (XRD). The simultaneous determination of quercetin (QR) and morin (MR) has been performed on glassy carbon electrode (GCE) modified with AgNPs-AETGO (AgNPs-AETGO/GCE). QR presented an oxidation step at Ea of 200mV and reduction step at Ec of 150mV and RT presented an oxidation step at Ea of 600mV at AgNPs-AETGO/GCE by cyclic voltammetry (CV). The linearity ranges and the detection limits of QR and MR were 1.0×10-8 - 5.0×10-6M and 3.3×10-9M, respectively. The AgNPs-AETGO/GCE was also applied to real samples for the simultaneous determination of QR and MR. Thus the developed method can be adopted as an alternative to the published chromatographic, spectrophotometric and electroanalytical methods for simultaneous determination of QR and MR.