Abstract
In dentistry, the wet-bonding approach relies on water to maintain demineralized collagen expanded for proper resin infiltration; nevertheless, hydrolytic instability of the resin-dentin interface is inevitable with current bonding techniques. Considering dimethyl sulfoxide’s (DMSO) ability to “biomodify” collagen and precipitate enzymes, the aim was to test whether the use of DMSO would permit adequate resin bonding to H3PO4-etched dehydrated dentin and assess its impact on collagen degradation by host-derived enzymes. Etched dentin surfaces from extracted sound human molars were randomly bonded in wet or dry conditions using aqueous or ethanolic DMSO solutions as pretreatments and bonding resins with or without DMSO. Bonded teeth were sectioned into resin-dentin slabs for confocal in situ zymography and beams for microtensile bond strength test. Demineralized powdered dentin was incubated in the tested DMSO -media and a hydroxyproline assay evaluated dissolution of collagen peptides. Zymography was performed on protein extracts obtained from dry and wet H3PO4-ecthed dentin powder treated with the DMSO- media. The correlative biochemical analysis demonstrated that reduction of water content during dentin hybridization by the innovative dry-bonding approaches with DMSO is effective to inactivate host-derived MMP-2 and MMP-9 and thus reduce collagen degradation while simultaneously optimizing resin-dentin bonding.
Highlights
Resin-dentin bonding is a revolutionary form of in situ tissue engineering in which intrinsically hydrated demineralized collagen[1] serves as a scaffold for resin infiltration to couple dental adhesives to the underlying mineralized dentin[2,3]
The tested null hypotheses were that: (i) dry-bonding using Dimethyl sulfoxide (DMSO) would have no impact on dentin bond strength; and (ii) the relative proteolytic activity of H3PO4 etched-dentin would not be affected by DMSO-pretreatments
While the conventionally accepted wet-bonding approach requires residual water to maintain demineralized collagen expanded for resin infiltration, the proposed DMSO-protocols produced improved resin bonding to dry dentin
Summary
Resin-dentin bonding is a revolutionary form of in situ tissue engineering in which intrinsically hydrated demineralized collagen[1] serves as a scaffold for resin infiltration to couple dental adhesives to the underlying mineralized dentin[2,3]. Incomplete[5] and suboptimal[6] resin infiltration, the inability of current bonding resins to completely replace free and loosely bound water within collagen matrix[7], hydrolytic instability of hydrophilic methacrylate monomers[8] and collagen degradation[2,9] remain as major drawbacks to the longevity of resin-dentin bonds[4]. Such processes can be considered as highly correlative since their resultant degradative effects occur exclusively in the presence of water[2]. The tested null hypotheses were that: (i) dry-bonding using DMSO would have no impact on dentin bond strength; and (ii) the relative proteolytic activity of H3PO4 etched-dentin would not be affected by DMSO-pretreatments
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
