Abstract
A novel double coating (DC) was developed for fast and reproducible microemulsion electrokinetic chromatography (MEEKC), as tested with separation and determination of amino acids using laser-induced fluorescence (LIF) detection after derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazol. The simple coating is a combination of a removable covalent layer and a dynamic SDS coating. Hexamethyldisilazane was utilized for the covalent layer that can be regenerated on-line. Compared with previous no-coating method, the analysis time was shortened; and the reproducibility of migration times was improved.
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