Abstract
In this study, a novel imprinted electrochemical sensor was constructed by modifying deoxyribonucleic acid (DNA) for the detection of Ag+ with high sensitivity and selectivity. Chitosan (CS) and Ag+ template ions were mixed and used to modify a glassy carbon electrode, forming a composite CS-Ag+ film. After CS-Ag+ film crosslinking, gold nanoparticles (AuNPs) electrodeposition, and removal of Ag+ templates, the imprinted sensor was prepared. A novel DNA-imprinted sensor was prepared by immobilizing Ag+ DNA aptamers on the surface of AuNPs. The electroactive area, surface morphology and electrochemical reaction mechanism of the DNA-imprinted sensor were analyzed. The basic steps of the reaction were studied through electrochemical reaction kinetics analysis. The experimental results show that the DNA-imprinted electrochemical biosensor can quantitatively detect Ag+ in the range of 10–50 nM (R2 = 0.99695) with a limit of detection (LOD) of 8.25 nM (3σ/slope). The sensitivity of the electrochemical biosensor was 1.7473 × 10−6 A/μM, and the effective area was 8.2121 cm2. The proposed novel DNA-imprinted sensor for detecting Ag+ has high sensitivity and selectivity.
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