Abstract
The absence of highly effective delivery systems is a major challenge for gene therapy. Our work was aimed at the development of novel cationic liposomes possessing high transfection efficiency. For this purpose, a novel disulfide polycationic amphiphile 2S4 was synthesized. Cationic liposomes based on 2S4 and a helper lipid DOPE were formed by the thin film hydration method and exhibited effective plasmid DNA delivery into the HEK293 cells, with a transfection activity superior to that of the commercial agent Lipofectamine® 2000. Our results suggest that the polycationic amphiphile 2S4 is a promising candidate for in vitro nucleic acid delivery.
Highlights
Gene therapy is an attractive tool for the treatment of both inherited [1,2] and acquired diseases [3,4] and is based on the delivery of therapeutic nucleic acid (NA) into cells
The most attractive and safe delivery vehicles are the non-viral ones, such as cationic liposomes (CLs). Despite their advantages, including safety, low cost, and the ability to be produced at scale, CLs have inadequate delivery efficiency [6]
It is known that the location of a disulfide bond delivery compared to Recently, we have developed a polycationic gemini amphiphile 2X3 with a hexamethylene location have strong effect on the transfection efficiency spacer and a acarbamoyl linker
Summary
Received: 22 January 2018; Accepted: 16 February 2018; Published: 18 February 2018
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