A Novel Disposable Immunosensor for Early Diagnosis of Cardiovascular Diseases

Abstract

AbstractCardiovascular diseases are highly common cause of mortality and morbidity. It is envisaged that there is a relationship between cardiovascular diseases and ST2 (Suppression of Tumorigenicity 2) concentration of protein. In patients with cardiac insufficiency, the ST2 concentration was reported as higher than people without cardiac insufficiency. In this study ITO‐PET sheets (indium tin oxide covered terephthalate) was used as a working electrode for the fabrication of the immunosensor for ST2 protein. In the optimization experiments, important parameters responsible for biosensor fabrication have been optimized. After determining optimal conditions, reproducibility, repeatability, regeneration possibility, and analytical properties such as storage life are determined. The immunosensor showed a linear detection range for ST2 between 1–1500 fg/mL with a LOD value of 3.98 fg/mL. We also carried out SFI (single frequency impedance) experiments to monitor the specific interaction between ST2 and anti‐ST2, simultaneously. The developed immunosensor was successively applied to the determination of ST2 in commercial human serum sample.