Abstract
Novel amorphous carbon nanoparticles (ACNPs) with high electrochemiluminescence (ECL) quenching efficiency were prepared from candle soot with a quite simple approach. The prepared ACNPs were employed as novel energy quenchers for an ECL immunosensor on a disposable screen-printed carbon electrode array (SPCEA) substrate. Gold nanoparticles-doped chitosan composite film was utilized to immobilize Ru(bpy)32+-labeled antibody on the SPCEA, and ACNPs were linked with antigens to obtain ACNP-antigen conjugate. The immunoreaction between the Ru(bpy)32+-labeled antibody and the ACNP-antigen conjugate bridged the Ru(bpy)32+ donor and the ACNPs acceptor, which led to quenching of ECL emission from Ru(bpy)32+via energy transfer. In the presence of the target antigen, this antigen competed with the ACNP-antigen for the immobilized Ru(bpy)32+-labeled antibody, thus led to decreased occurrence of the ECL quenching. This ACNPs-based ECL quenching system was successfully applied to detect mouse IgG, which was used as a model analyte. The method showed a linear range of 0.50–400ngmL−1 and a detection limit of 0.35ngmL−1. The results for real sample assay and recovery test demonstrated the reliability of this ECL quenching-based immunosensor. It could be further extended to multiplexed immunoassay of biomarker proteins for clinical diagnosis.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
