Abstract
The current research focused on detecting the role of Salmonella typhi (S. typhi) in the formation of gallbladder stones in the laboratory following isolation and diagnosis of S. typhi from bile samples of patients suffering from gallstone. Locally and for the first time, a novel continuous culture was designed, and Brilliant Green Bile Broth (BGBB) was used by adding 60% cholesterol and 20% Calcium Carbonate (CaCO3) to form the gallstone nucleation. The continuous culture was inoculated with 1% S. typhi then incubated in optimal conditions for 20 days. After 14 days of incubation, results revealed the formation of spherical aggregations with various sizes in the test flask compared to the control flask. Moreover, an increase in the size of the stone formed was observed after 20 days of incubation. The morphology of cholesterol and Calcium Carbonate crystals were studied using light, fluorescent and scanning electron microscopes, and the functional groups were diagnosed using the Fourier transform infrared spectrometry (FTIR) technique. KEYWORDS Biliary microbiota, model Bile, polysaccharide production
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Basic and Applied Sciences - Scientific Journal of King Faisal University
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
