A Novel Design and Fabrication of Ascorbic Acid Sensitive Biosensor Based on Combination of HAP/rGO/AuNPs Composite and Ascorbate Oxidase

Abstract

In this study, a new HAP/rGO/AuNPs-AO modified carbon electrode (GCE) was fabricated for amperometric detection of ascorbic acid. The HAP/rGO/AuNPs composite was prepared by one-step hydrothermal method. The large specific surface area, strong adsorption and excellent biocompatibility of HAP nanowires enabled fast, stable and accurate detection of ascorbic acid. As array of quantum dot, AuNPs had strong electrocatalytic activity in addition to good adsorption to enzymes, which could provide high sensitivity and low limit of detection for biosensor. Then rGO ensured that current signal could be quickly transferred from the composite to electrode. Under the optimum conditions, the prepared biosensor demonstrated a wide linear range from 0.39 to 36 mM (R2 = 0.99845), a low limit of detection (3.39 μM, S/N = 3) and a high sensitivity (15.949 mA moL−1) with good selectivity, stability (10.8%) and repeatability (3.9%), which might providing a reasonable and reliable way for ascorbic acid detection.