Abstract
Cytosolic components of human neutrophils, p47 phox and p67 phox , deficiencies of which lead to chronic granulomatous disease (CGD), potentiate respiratory burst oxidase translocating from cytosol to membrane upon cell stimulation. In this report we describe a novel cytosolic component, p40 phox , which consistently behaves with p67 phox through immunoprecipitation and column works, and is missing in patients with CGD who lack p67 phox . Although actin has been reported to be involved in O 2 − generation, the p40 phox profile did not correspond to that of actin. The tight association between p40 phox and p67 phox was not affected by treatment with a mixture of deoxycholate and Nonidet P-40, until subjected to SDS-PAGE. Addition of recombinant p67 phox to cytosol did not produce any additional p40 phox in the immunoprecipitate, unlike the additive increment in the band of p67 phox . These results suggest that p40 phox forms a complex with p67 phox in a molar ratio of 1:1, without any free p40 phox in the cytosol.
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More From: Biochemical and Biophysical Research Communications
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