A Novel Composite and Suspended Nanofibrous Scaffold for Skin Tissue Engineering

Abstract

Electrospinning is a technique for creating continuous nanofibrous networks that can architecturally be similar to the structure of extracellular matrix (ECM). In this work, a kind of PLGA/silk fibroin composite electrospinning nanofibrous scaffolds is proposed for the growth of skin cells in combination with the good mechanical properties with not easy to deformation of silk fibroin and the self-floating of PLGA. The SEM experiment of the silk fibroin/PLGA composite nanofibers indicated that the prepared nanofibers are smooth, uniform in size and have good porosity. The floating experiment is shown that this scaffold is able to suspend in water and is also able to suspend in cell culture medium, hence, the skin cells seeded on the scaffold are exposed to air as required in skin tissue engineering. The wrinkled contrast experiment and the degradation experiment are verified the usefulness of this scaffold.