Abstract
Hypochlorite (ClO−) is a well-known redox regulator among reactive oxygen species and plays a crucial role in bacterial disinfection and body immunity. Therefore, there is a high demand for an analytical technique to monitor ClO− under physiological conditions. Herein, a novel fluorescence probe, RES-DPPT, was developed for detecting ClO−. This probe employs diphenylphosphinothioyl unit as the recognition group and resorufin as the fluorescent platform. RES-DPPT has high sensitivity and specificity, rapid response, and wide pH tolerance for monitoring ClO−. Additionally, RES-DPPT undergoes colorimetric and fluorescence color changes in the solution, which can be observed by the “naked eye.” Furthermore, RES-DPPT is successfully able to detect exogenous and endogenous ClO− in living cells.
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