Abstract
Abstract When developing cell lines for the production of antibodies, it is essential to choose healthy clones with high levels of IgG per cell. This process typically requires several assays on multiple platforms to get enough information to select optimal clones. Due to the expense and effort involved, this limits the number of clones that can be evaluated. We have developed the Cy-Clone™ PLUS Kit, a rapid, no-wash screening assay that simultaneously provides IgG concentration, IgG concentration per cell and per viable cell, cell viability, and cell density in every well of a 384-well plate. CHO cells transfected with human IgG genes were inoculated, plated by limiting dilution, and cultured for 20 days. Cells were mixed with the reagents from the Cy-Clone PLUS kit and then read on the iQue® Screener PLUS platform in ~22 minutes. Using ForeCyt® Software, readouts for each well were visualized using heat maps and Profile Map to identify wells meeting multiple IgG production criteria. The Cy-Clone PLUS kit with the iQue Screener platform provides all the relevant data needed to select and rank the best clones in a single well without screen-size limitations. The Cy-Clone PLUS kit enables you to make more informed decisions earlier, shorten development time, and decrease costs.
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