Abstract
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Highlights
In the last decade a novel class of growth factors related to the herpesvirus group was characterized which substantially differ from other virus-related growth factors which represent viral versions of known cellular growth factors
Isolation and basic characteristics of herpesvirus related growth factors Growth factors related to herpesviruses could be detected either in virus transformed cells, e.g. pseudorabies virus (PRV) related growth factor (PRGF), which was originally obtained from PRV transformed HPR-1 cells, or in cells infected with virus at low MOI and cultivated in conditions which are non-permissive for replication of virus
Growth factors associated with herpesviruses (HVGFs) such as PRGF, or HSGF-2 could be obtained from virus free medium of infected MK-2 cells (Ga‰perík et al 1996)
Summary
The direct role of some alpha herpesviruses in the formation of malignant tumours is questionable, if at all, there exists a body of evidence for their oncogenic potential in vitro (Rapp and Reed 1976; Hampar 1981), even though the mechanisms of transformation are still unknown (Galloway and McDougall 1981; 1990). Isolation and basic characteristics of herpesvirus related growth factors Growth factors related to herpesviruses could be detected either in virus transformed cells, e.g. PRV related growth factor (PRGF), which was originally obtained from PRV transformed HPR-1 cells, or in cells infected with virus at low MOI and cultivated in conditions which are non-permissive for replication of virus. MK-2 cells derived from monkey kidney (Hull et al 1962) were shown to be the best producers of all tested growth factors (Ga‰perík et al 1996) Virus replication in these cells is limited, only the trace amounts of infectious virus are shed into the culture medium, which can be successfully removed lowering pH as mentioned above
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