Abstract
Histidine-containing microspheres (HCM) with 2 μm in size were synthesized by suspension polymerization of poly(hydroxyethyl methacrylate) and N-methacryloyl-L-histidine methyl ester. Then, they were used to prepare composite cryogel columns by an embedding process for affinity depletion of immunoglobulin G (HIgG) from human serum via histidine groups on microspheres. Here, we describe HIgG adsorption performance of composite cryogel columns in both aqueous solution and human serum.
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