Abstract

Stearic acid (SA) grafted chitosan oligosaccharide (CSO) (CSO–SA), which was synthesized by an 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)-mediated coupling reaction, was demonstrated to form micelle like structure by self-aggregation in aqueous solution. The critical micelle concentration (CMC) of CSO–SA with 15.4% amino substituted degree of CSO was about 0.035 mg/ml. The micelles with 1 mg/ml CSO–SA concentration had 70.6 nm volume average hydrodynamic diameter with a narrow size distribution and 46.4 ± 0.1 mV surface potential. Due to the cationic property, the micelles could compact the plasmid DNA to form micelle/DNA complexes nanoparticles, which can efficiently protect the condensed DNA from enzymatic degradation by DNase I. The volume average hydrodynamic diameter of CSO–SA micelle/DNA complex increased from 203 nm to 318 nm and decreased to 102 nm due to the variation of zeta potential when the N/P ratio increased from 0.25 to 3.6 and from 3.6 to 58. The IC 50 value of the CSO–SA micelle against A549 cells was 543.16 μg/ml, while the IC 50 of Lipofectamine™ 2000 was about 6 μg/ml. The in vitro transfection efficiency of CSO–SA micelles was investigated by using plasmid DNA (pEGFP-C1). The transfection efficiency with CSO–SA/DNA (N/P ratio is 29) was increased with the post-transfection time (in 76 h), while the optimal transfection of Lipofectamine™ 2000/DNA was obtained at 24 h. The transfection of CSO–SA was not interfered in the presence of 10% fetal bovine serum, which showed remarkable enhancement effect. The optimal transfection efficiency of CSO–SA micelles in A549 cells was about 15%, which was higher than that of CSO (about 2%) and approach to that of Lipofectamine™ 2000 (about 20%). The low cytotoxic biodegradable CSO–SA micelles could be used as an effective DNA condensation carrier for gene delivery system.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call