A novel chemiluminescence reaction system for the determination of norfloxacin with Ag(III) complex

Abstract

A novel chemiluminescence (CL) system for the determination of norfloxacin (NFLX) is developed based on the direct CL reaction of [Ag(HIO 6) 2] 5−–H 2SO 4–NFLX system. The possible mechanism of CL emission and enhancing effect was discussed by comparing UV, fluorescence and CL spectra. [Ag(HIO 6) 2] 5− in the presence of H 2SO 4 could produce CL emission at 490 nm, this might be caused by the excited state (O 2) 2*. The enhancing effect of NFLX may be produced through an intermolecular energy transfer from part of (O 2) 2* to NFLX molecule and complex of Ag 3+ and NFLX. The CL intensity emission intensity was linear in the range 1.34 × 10 −8 to 5.44 × 10 −6 g mL −1 with correlation coefficient of 0.9982. The detection limit (s/n = 3) was 3.10 × 10 −9 g mL −1. The recovery was in the range of 90.0–104% with the RSD of 1.1–2.8%. The proposed flow injection CL method was applied satisfactorily for the determination of NFLX in capsule, human serum and urine.