Abstract
Objectives: The purpose of the study is to evaluate the demineralizing efficacy of combined L(+) Lactic Acid And D-Gluconic Acid (LAGA) solution and combined yogurt and honey on human specimens of calcific valves and coronaries retrieved from patients who underwent aortic valve replacement, mitral valve replacement and heart transplantation. Background: Cardiovascular calcification is a major health problem in the aging population and hence, the need for therapeutic strategies to attenuate the disease process. Methods: Serial sections of 4 Formalin Fixed Paraffin Embedded (FFPE) calcific aortic valves, 4 calcific mitral valves and 12 coronary arteries were treated with LAGA solution. Von Kossa silver staining was used for histological semiquantitative evaluation of extent of calcification. A direct treatment of the tissue specimens with combined yogurt and honey was also performed. Results: A complete dissolution of the calcification was noted in all the aortic, mitral and coronary specimens after treatment with LAGA solution (P<0.0001). A direct treatment of the aortic, mitral, and coronary specimens with combined yogurt and honey for 24 hours showed mild reduction in the degree of calcification by at least one grade that was statistically significant (P<0.01). Conclusions: LAGA solution is efficacious in demineralizing calcific aortic and mitral valves and calcific coronary arteries. Combined yogurt and honey result in mild reduction in calcification. Our study may pave the way for epidemiological studies and potential therapeutic strategies to treat patients with vascular calcification.
Highlights
Calcific degenerative valvular heart disease is a major health problem in industrialized nations, affecting older populations
Lactic Acid And D-Gluconic Acid (LAGA) solution is efficacious in demineralizing calcific aortic and mitral valves and calcific coronary arteries
Our study may pave the way for epidemiological studies and potential therapeutic strategies to treat patients with vascular calcification
Summary
Calcific degenerative valvular heart disease is a major health problem in industrialized nations, affecting older populations. The prevalence of degenerative valvular heart disease increases with age, reported in 13% of patients older than 75 years of age and is associated with a worse survival [1]. The Cardiovascular Health Study was the first to describe the risk factors associated with calcific aortic valve disease including age, smoking, male gender, hypertension, and an elevated low-density lipoprotein, similar to those of atherosclerosis [2]. The recent emerging evidence for active regulation of proosteogenic signaling in advanced aortic valve has led to a major paradigm shift in the understanding of pathogenesis of calcification from a passive degenerative process to an actively regulated one [4]. Cardiovascular calcification is a major health problem in the aging population and the need for therapeutic strategies to attenuate the disease process
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