Abstract
Screening and obtaining a novel high activity cellulase and its producing microbe strain is the most important and essential way to improve the utilization of crop straw. In this paper, we devoted our efforts to isolating a novel microbe strain which could produce high activity cellulase. A novel strain Trichoderma virens ZY-01 was isolated from a cropland where straw is rich and decomposed, by using the soil dilution plate method with cellulose and Congo red. The strain has been licensed with a patent numbered ZL 201210295819.6. The cellulase activity in the cultivation broth could reach up to 7.4 IU/mL at a non-optimized fermentation condition with the newly isolated T. virens ZY-01. The cellulase was separated and purified from the T. virens culture broth through (NH4)2SO4 fractional precipitation, anion-exchange chromatography and gel filtration chromatography. With the separation process, the CMC specific activity increased from 0.88 IU/mg to 31.5 IU/mg with 35.8 purification fold and 47.04% yield. Furthermore, the enzymatic properties of the cellulase were investigated. The optimum temperature and pH is 50 °C and pH 5.0 and it has good thermal stability. Zn2+, Ca2+ and Mn2+ could remarkably promote the enzyme activity. Conversely, Cu2+ and Co2+ could inhibit the enzymatic activity. This work provides a new highly efficient T. virens strain for cellulase production and shows good prospects in practical application.
Highlights
With the development of agriculture, cellulose-rich straws from cropland have become one of the largest amounts of biomass
If biological resources that are prevented from being directly burned in cropland can be utilized as carbohydrates, the atmospheric pollution can be avoided and a huge amount of carbohydrates, such as fermentable sugars, can be provided
Cellulose could be efficiently degraded to fermentable sugars with conventional biological processes
Summary
With the development of agriculture, cellulose-rich straws from cropland have become one of the largest amounts of biomass. They should be comprehensively utilized as biological resources. If biological resources that are prevented from being directly burned in cropland can be utilized as carbohydrates, the atmospheric pollution can be avoided and a huge amount of carbohydrates, such as fermentable sugars, can be provided. Cellulose could be efficiently degraded to fermentable sugars with conventional biological processes. A high activity of cellulase is the key factor to this biological process of straw utilization as biological resources [5,6,7]. Cellulase has a very broad application in other fields such as food processing, oil extraction, agricultural industries, brewery, animal feed, textile and other fields like laundry, pulp, paper, detergent industry [8,9,10,11,12]
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