A novel cell-based assay for measuring neutralizing autoantibodies against type I interferons in patients with autoimmune polyendocrine syndrome type 1.

Abstract

Event Abstract Back to Event A novel cell-based assay for measuring neutralizing autoantibodies against type I interferons in patients with autoimmune polyendocrine syndrome type 1. Lars Breivik1*, Bergithe E. Oftedal1, Anette S. Wolff1, Elizaveta Orlova2 and Eystein S. Husebye1, 3 1 University of Bergen, Department of Clinical Science, Norway 2 Endocrinology Research Center, Institute of Paediatric Endocrinology, Russia 3 Haukeland University hospital,, Department of Medicine, Norway Autoimmune polyendocrine syndrome type 1 (APS-1) is a unique monogenic disorder caused by loss-of-function mutations in the autoimmune regulator (AIRE) gene. The loss of a functional AIRE protein presumably causes defects in central thymic tolerance and the escape of pathogenic autoreactive T cells into peripheral tissues. The most prevalent clinical manifestations are chronic mucocutaneous candidiasis, hypoparathyroidism and primary adrenocortical failure although a wide range of other autoimmune manifestations may occur. An important characteristic feature of APS-1 is the presence of neutralizing serum autoantibodies against type I interferons (subtypes –α and -ω) at frequencies approaching 100 %. These autoantibodies may therefore serve as an important diagnostic tool, complementing genetic mutation analysis of AIRE when APS-1 is suspected. Several techniques for measuring these antibodies are currently available, including fluid-phase binding assays using recombinant antigens labeled with radioactivity (radioactive immunoassay; RIA) or luciferase, and assays measuring the actual interferon-neutralizing capabilities of the antibodies such as the antiviral interferon neutralizing assay (AVINA). We report here on a cell-based assay using a commercially available reporter cell-line that provides a simple, sensitive, rapid and reliable way of assaying neutralizing autoantibodies against the type I interferons subtypes –α and –ω as compared to a RIA assay. Acknowledgements We would like to thank all the doctors and patients that contributed to this work. The technical help from Elisabeth T. Halvorsen is greatly acknowledged. The study has been supported by grants from Helse-Vest and The Norwegian Research Council. Keywords: Autoantibodies, Interferon Type I, Assay development, APS-1, biomarker Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Breivik L, Oftedal BE, Wolff AS, Orlova E and Husebye ES (2013). A novel cell-based assay for measuring neutralizing autoantibodies against type I interferons in patients with autoimmune polyendocrine syndrome type 1.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00920 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Lars Breivik, University of Bergen, Department of Clinical Science, BERGEN, N-5021, Norway, larsbreivik@hotmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Lars Breivik Bergithe E Oftedal Anette S Wolff Elizaveta Orlova Eystein S Husebye Google Lars Breivik Bergithe E Oftedal Anette S Wolff Elizaveta Orlova Eystein S Husebye Google Scholar Lars Breivik Bergithe E Oftedal Anette S Wolff Elizaveta Orlova Eystein S Husebye PubMed Lars Breivik Bergithe E Oftedal Anette S Wolff Elizaveta Orlova Eystein S Husebye Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.