Abstract
Two non-electroactive biomarkers methylglyoxal (MGo) and glyoxal (Go) in urine and environmental water samples were determined for the first time by capillary electrophoresis with amperometric detection (CE-AD) after derivatizing with an electroactive compound 2-thiobarbituric acid. Experimental conditions of derivatization and CE-AD detection were optimized. Highly linear response was obtained for these two biomarkers over three orders of magnitude with good correlation ( r 2 > 0.999). The limits of detection (LODs) and limits of quantitation (LOQs) of MGo and Go were 0.2 μg L −1 and 1.0 μg L −1, 0.5 μg L −1 and 2.0 μg L −1, respectively. The average recovery and relative standard deviation (RSD) were within the range of 90.9–101.3% and 0.7–2.2%, respectively. The proposed CE-AD method provides a reliable and sensitive quantitative evaluation of MGo and Go in real sample matrices by employing relatively simple and inexpensive instrument.
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