Abstract
As polysaccharides of the mushroom genus Pleurotus can act as biological response modifiers, we now describe the chemical characterization of a new water-soluble αβ-glucan from the fruiting bodies of the edible mushroom Pleurotus florida. It was obtained via successive aq. extraction, freeze-thawing, precipitation with Fehling solution, and dialysis through a membrane with M r 1000 kDa cut-off. It was analyzed using NMR spectroscopy, HPSEC, monosaccharide composition, methylation analysis, and a controlled Smith degradation. It was a branched glucan ( M w 1.1 × 10 6 g/mol), with a main-chain of (1→3)-linked-α- d-Glc p residues, partially substituted at O-6 by single-unit β- d-Glc p, and a minor proportion by side-chains with 3- O-substituted β- d-Glc p groups.
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