Abstract
This study aimed to examine the optimal cross-link density of recombinant peptide (RCP) particles, based on human collagen type I, for bone reconstruction in human alveolar cleft. Low- (group 1), medium- (group 2), and high- (group 3) cross-linked RCP particles were prepared by altering the duration of the heat-dependent dehydration reaction. Rat palatine fissures (n = 45), analogous to human congenital bone defects, were examined to evaluate the potential of bone formation by the three different RCP particles. Microcomputed tomography images were obtained to measure bone volume and bone mineral density at 4, 8, 12, and 16 weeks post grafting. Specimens were obtained for histological analysis at 16 weeks after grafting. Additionally, alkaline phosphatase and tartrate acid phosphatase staining were performed to visualize the presence of osteoblasts and osteoclasts. At 16 weeks, bone volume, bone mineral density, and new bone area measurements in group 2 were significantly higher than in any other group. In addition, the number of osteoblasts and osteoclasts on the new bone surface in group 2 was significantly higher than in any other group. Our results demonstrated that medium cross-linking was more suitable for bone formation—and could be useful in human alveolar cleft repairs as well.
Highlights
Bone reconstruction of the alveolar cleft is one of the most difficult and challenging alveolar bone defect repairs
This study evaluated the use of recombinant peptide (RCP) particles as a prospective therapeutic approach for bone reconstruction to treat human alveolar cleft, a congenital defect
This study was the first experiment in which RCP particles were grafted into a rat palatine fissure and bone formation was evaluated
Summary
Bone reconstruction of the alveolar cleft is one of the most difficult and challenging alveolar bone defect repairs. Autologous bone graft is widely regarded as the gold standard for this purpose [1,2,3,4]. It has several drawbacks, including pain at the donor site, development of deformity, possible injury to adjacent anatomical structures, and loss of grafted bone [5,6]. Type I collagen is the principal component of the organic matrix of bone, as well as of other connective tissues [8,9]. Type I collagen-based bone substitute has received significant attention in the field of bone tissue engineering [10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
