A novel bocavirus in canine liver

Linlin Li,Lark L Coffey,Marko Estrada,Chunlin Wang,Samia N Naccache,Xutao Deng,Erik Samayoa,Christian M Leutenegger,Eric Delwart,Jianming Qiu,Patricia A Pesavento,Charles Chiu

doi:10.1186/1743-422x-10-54

Abstract

BackgroundBocaviruses are classified as a genus within the Parvoviridae family of single-stranded DNA viruses and are pathogenic in some mammalian species. Two species have been previously reported in dogs, minute virus of canines (MVC), associated with neonatal diseases and fertility disorders; and Canine bocavirus (CBoV), associated with respiratory disease.FindingsIn this study using deep sequencing of enriched viral particles from the liver of a dog with severe hemorrhagic gastroenteritis, necrotizing vasculitis, granulomatous lymphadenitis and anuric renal failure, we identified and characterized a novel bocavirus we named Canine bocavirus 3 (CnBoV3). The three major ORFs of CnBoV3 (NS1, NP1 and VP1) shared less than 60% aa identity with those of other bocaviruses qualifying it as a novel species based on ICTV criteria. Inverse PCR showed the presence of concatemerized or circular forms of the genome in liver.ConclusionsWe genetically characterized a bocavirus in a dog liver that is highly distinct from prior canine bocaviruses found in respiratory and fecal samples. Its role in this animal’s complex disease remains to be determined.

Highlights

Bocaviruses are classified as a genus within the Parvoviridae family of single-stranded DNA viruses and are pathogenic in some mammalian species
We genetically characterized a bocavirus in a dog liver that is highly distinct from prior canine bocaviruses found in respiratory and fecal samples
Phylogenetic analysis of the entire VP1 was performed to determine the relationship between Canine bocavirus 3 (CnBoV3) and other bocaviruses

Summary

Introduction

Bocaviruses are classified as a genus within the Parvoviridae family of single-stranded DNA viruses and are pathogenic in some mammalian species. Two species have been previously reported in dogs, minute virus of canines (MVC), associated with neonatal diseases and fertility disorders; and Canine bocavirus (CBoV), associated with respiratory disease. Bocaviruses are known to infect multiple mammalian species including humans [2], cows [3], pigs [4,5,6], gorillas [7], chimpanzees [8], California sea lions [9], dogs [10,11,12,13], cats [11], bats [14], and pine martens [15]. Minute virus of canines (MVC) was the first known bocavirus infecting dogs. MVC was isolated in 1967 in the feces of a clinical healthy dog, and later recognized

Methods

Results

Conclusion