A novel biopolymer technique for encapsulation of Bacillus velezensis BV9 into double coating biopolymer made by in alginate and natural gums to biocontrol of wheat take-all disease

Abstract

Bacillus velezensis has been known for its high potential in controlling agricultural diseases. Technological advances have opened new perspectives for producing effective formulations by reducing some of the obstacles to their use, such as instability and loss of activity due to exposure to adverse environmental conditions. Encapsulation is one of the new approaches in agricultural science. This research describes discoveries related to processes for the microencapsulation of B. velezensis with natural gums. The efficiency, survival, and controlled release of B. velesensis BV9 encapsulated with alginate mixed with zedo gum, mastic gum, and tragacanth gum were evaluated for this aim. Furthermore, under greenhouse conditions, the encapsulated cells were assessed to control Gaeumannomyces graminis var. tritici in wheat. The results indicated that all tested microcapsules protected >60 % of the bacterial cells. The Alginate-Zedo Gum (Alg-ZG) microcapsules showed a better-controlled release over two months. The greenhouse study indicated that treating wheat plants with Alg-ZG microcapsules was the most efficient treatment, suppressing 100 % of the pathogen. The results indicated that Alg-ZG is the most promising mixture to improve the survivability of B. velezensis BV9. Also, using natural gums and great potential of this formulation provides an effective and affordable fertilizers for agriculture.