Abstract
l-Fructose, which was produced from l-psicose using immobilized d-tagatose 3-epimerase, was utilized as a starting material in the preparation of an uncommon aldose-hexose, l-glucose, by cell reaction. A mutant strain, Klebsiella pneumoniae strain 40bXX, produced d-arabinose isomerase constitutively. Toluene-treated cells of the mutant strain, which were used as the source of crude d-arabinose isomerase, were employed in the conversion of l-fructose to l-glucose. Empirically, 0.35 g of l-glucose was obtained from 1.0 g of l-fructose, viz an overall yield of 35%. The product obtained was purified and identified to be l-glucose by high performance liquid chromatography (HPLC) analysis, and was ultimately confirmed by 13C nuclear magnetic resonance (13C NMR) spectra.
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