Abstract
The work carried out on Shimadzu LC-2009 SgimadzuHPLC system equipped with Thermo C18 (150 �?� 4.6 mm,5�?�) column with mobile phase containing 10 mMpotassium dihydrogen phosphate buffer, pH 6.4: ACN: Water in the ratio 20:70:10 v/v/v (1 ml/min.) and detectionwavelength 231 nm. The retention time of Amlodipine besylate was found to be 4.1 min. The developed bioanalytical method was found to be linear in concentration range of 24-48 ng/ml (R2=0.9913). The precision study revealed that the percentage cumulative variation was within acceptable limit and accuracy study showed the value of mean percent recovery between 101.61%-102.20%. The Amlodipine besylate was stable in human plasma at different storage conditions. The validation parameters of the method met the acceptance criteria. Sufficient stability of both LQC and HQC was shown to allow for completion of sample analysis in clinical trials. From the results, we can conclude that developed bioanalytical method can be used for routine analysis of Amlodipine besylate. A simple, sensitive, Rapid and precise bioanalytical RP-HPLC method was developed for estimation of Amlodipine besylate in human plasma.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: International Journal of Drug Development and Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
