Abstract
Our inability to accurately monitor individual neurons and their synaptic activity precludes fundamental understanding of brain function under normal and various pathological conditions. However, recent breakthroughs in micro- and nano-scale fabrication processes have advanced the development of neuro-electronic hybrid technology. Among such devices are three-dimensional and planar electrodes, offering the advantages of either high fidelity or longer-term recordings respectively. Here, we present the next generation of planar microelectrode arrays with “nano-edges” that enable long-term (≥1 month) and high fidelity recordings at a resolution 15 times higher than traditional planar electrodes. This novel technology enables better understanding of brain function and offers a tremendous opportunity towards the development of future bionic hybrids and drug discovery devices.
Highlights
Our inability to accurately monitor individual neurons and their synaptic activity precludes fundamental understanding of brain function under normal and various pathological conditions
The three-dimensional electrodes tend to allow for high fidelity recordings they only do so over a short time period
We found the neuronal coupling coefficient to be 0.15, which is 15 times higher than what has been reported for traditional planar and resistor electrodes[10] (0.001–0.011)
Summary
Inspired by the morphology of a synaptic cleft, whereby both pre- and postsynaptic structures are juxtaposed and semi-encapsulated, we developed microelectrodes mimicking a synapse morphology as well as neuronal juxtaposition with their adjacent cells. Microelectrodes that “bio-mimic” the postsynaptic cleft were designed to exhibit ‘nano-edges’ that provide a tighter physical and dielectrical seal between the device and the neuron This structural geometry was anticipated to prevent the leakage of current into the surrounding extracellular milieu, preserving and augmenting the functional integrity of chemical and electrical neuronal signal processing (Fig. 1a,b). As demonstrated by the standard deviation, the observed variability is due to numerous application specific factors Chief among these are cell-specific variables such as the size of the neurons and the exact interfacing between their membrane and the electrode which enables the nano-edge to fully increase the sealing resistance. The sealing resistances calculated using this model for a nano-edge microelectrode of 30 μmin diameter ranged from 0.66 MΩto 8.71 MΩ,depending on the height of the nano-edge and the size of the neuron simulated.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
