A novel aptasensor for colorimetric monitoring of tobramycin: Strategy of enzyme-like activity of AuNPs controlled by three-way junction DNA pockets

Abstract

In this study, a novel colorimetric sensor was introduced to detect tobramycin (TOB) based on controlling the catalytic activity of gold nanoparticles (AuNPs) by the three-way junction aptamer pockets. In the absence of TOB, the surfaces of AuNPs were masked by the three-way junction pockets that prevented their catalytic activation for the reduction of 4-Nitrophenol in the presence of NaBH4. While the formation of the pockets was prevented in the presence of TOB that facilitated the 4-Nitrophenol access to AuNPs. Hence, the catalytic reduction of 4-Nitrophenol induced a color change of the solution from yellow to colorless, highlighting the presence of the target. The aptasensing assay provided good target specificity with a detection limit (LOD) of 1.16 µM and a linear dynamic range over 4–32 µM. The aptasensor was successfully applied to quantitatively monitor TOB in the human serum and milk samples with the LODs of 1.38 and 1.42 µM and recovery values of 94.87–105.75% and 93.75–105.31%, respectively.