A novel approach to produce glucose from the supernatant obtained upon the dilute acid pre-treatment of rice straw and synergistic action of hydrolytic enzymes producing microbes.

Abstract

The present work refers to a process involving the use of dilute nitric acid pretreatment and enzymatic hydrolysis for the transformation of rice straw into simple sugars. Acid pre-treated rice straw was separated into the pulp and supernatant through centrifugation and filtration. The two fractions are then converted into simple sugars by combined action of microbes producing cellulase and laccase enzymes. These microbes were isolated from soil samples which were collected from different locations with varying altitudes, expected to harbour microbes with high-hydrolysing activity. The nitric acid pretreatment was carried out at 30°C, 200rpm for 72h. After 72h, the culture supernatants were analysed for the presence of glucose with the help of HPLC. The supernatant fraction separated after the acid pre-treated rice straw produced highest amount of glucose (205mg/g of rice straw) upon subsequent hydrolysis with synergistic action of cellulase and laccase-producing microbes.