Abstract
A simple, sensitive, and selective method based on the fluorescence quenching of BSA-stabilized gold nanoclusters (BSA-AuNCs) for rapid determination of vitamin B12 is developed. The fluorescence emission was measured at λex/λem 370/617 nm in phosphate buffer solution of pH 7.4, and the experimental variables and possible interference were studied. Under optimized conditions, the proposed method allows the determination of vitamin B12 over the range 160.0 ng mL−1 to 38.5 μg mL−1 with a correlation coefficient of 0.998 and a limit of quantification of 100.0 ng mL−1. The proposed method was successfully applied to commercial injection dosage forms. The recovery of the method is in the range of 97.7–102.0% and the relative standard deviation is in the range of 2.0%–5.9%.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
