Abstract
Background Characterization of allergen epitopes is an essential task in unraveling the molecular mechanisms of allergy. At present, the procedures to characterize conformational epitopes are relatively laborious and, consequently, knowledge of conformational allergen epitopes is limited. The aim of this study was to couple an established epitope mapping approach, based on phage-displayed peptide libraries, with high-throughput sequencing in order to make conformational epitope mapping less laborious and increase data output.
Highlights
Characterization of allergen epitopes is an essential task in unraveling the molecular mechanisms of allergy
The aim of this study was to couple an established epitope mapping approach, based on phage-displayed peptide libraries, with high-throughput sequencing in order to make conformational epitope mapping less laborious and increase data output
The most abundant peptide sequences from round 3 were mapped to the same area on ovalbumin by two separate computer-based algorithms, suggesting a potential conformational epitope
Summary
Characterization of allergen epitopes is an essential task in unraveling the molecular mechanisms of allergy. The procedures to characterize conformational epitopes are relatively laborious and, knowledge of conformational allergen epitopes is limited. The aim of this study was to couple an established epitope mapping approach, based on phage-displayed peptide libraries, with high-throughput sequencing in order to make conformational epitope mapping less laborious and increase data output
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