A novel approach and a fully automated microcomputer‐based system to study kinetics of NO−3, NO−2, and NH+4 transport simultaneously by intact wheat seedlings

Abstract

Abstract A 16‐channel fully automated microcomputer‐based system was designed to measure the disappearance of NO−3 NO−2 and NH+4 simultaneously from uptake solutions. The analyses were done using high‐performance liquid chromatography. Statistical procedures were used to generate transport kinetics and interactions amongst NO−3, NO−2 and NH+4 by intact wheat seedlings. The simultaneous analysis of NO−3, NO−2 and NH+4 at real‐time; the accommodation of varying sampling intervals; the capability to study up to 16 experimental units in synchrony; and the analysis of the data with a microcomputer, make this a powerful system for studying transport kinetics and interactions.