Abstract
Objective: The purpose of current study was to investigate effects of application of Wutou and Banxia aqueous extract in the wound rats. Methods: Rats were fulfilled a surgical lesion with a 2.0 cm resecting tissue in the dorsal fascia. Following, animals were divided into 3 groups, including model group, control group treated with 1 mg/mL of Yunnan Baiyao, and Wutoubanxia group administrated 1 mg/mL of Wutou and Banxia extract. Wound contractions in day 0, 3, 7, 11 were calculated by an image analyzer. The histological analysis was detected using hematoxilin and eosin. The levels of tumor necrosis factor α (TNF-α), interleukin-2 (IL-2), transforming growth factor-β1 (TGF-β1), and basic Fibroblast Growth Factor (bFGF) transcripts in the wound tissue were determined by real-time quantitative PCR. Results: Compared with the control group, rats in the model group showed poor re-modeling and re-epithelization characterized by a significant decrease of neovascularization, epithelialization and fibroblast. Furthermore, the expression levels of TNF-α, IL-2 were significantly increased, and TGF-β1 and bFGF significantly decreased in the model group in contrasted with that in the control group. By contrast, the treatment of Wutoubanxia extract reversed the above-mentioned conditions caused by wound. Conclusion: The results suggest that administration of Wutou and Banxia extract has a promoting role in wound healing of rats possibly through enhancing anti-inflammatory ability and inducing fibroblast formation.
Highlights
In the resent years, wounds showed a gradually elevating-trend, and perhaps last for this status in the future with respect of unpredictable of natural disasters, increasing traffic accidents, abruptly physical injuries and so on [1,2]
Effects of Wutou and Banxia extract on wound healing in excisional rat Animals contractions in the Wutoubanxia treated groups were significantly increase from day 3 to 7 at a level 98.61%, day 7 to day 11 at 47.73%, and the complete wound healing occurred at day 11 compared with that of the model group
Faster keratinization characterized with minor intraepithelial cornification was occurred in the control group and the Wutoubanxia treatment group
Summary
Wounds showed a gradually elevating-trend, and perhaps last for this status in the future with respect of unpredictable of natural disasters, increasing traffic accidents, abruptly physical injuries and so on [1,2]. Process of wound healing is divided into three overlapping phases: inflammation (0-3 days), cellular proliferation (312 days), and remodeling (3-6 months) [6,7,8]. The fibroblasts facilitate the formation of granulation tissue, proliferation moves into the wound, causing extracellular matrix production, connective tissue fibers and neovessels [8]. A new therapeutic candidate with more efficiency applied in the two stages should have a direct impact on the wound healing [9,10,11]. It is exciting that great progresses have achieved along application of herbs in the wound healing [12,13,14,15]
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