A novel application of breath hydrogen and methane monitoring in nutritional science

Abstract

s / Journal of Nutrition & Intermediary Metabolism 1 (2014) 1e55 14 I296V; rs10246939) and grouped by haplotype. Serum and red cell folate levels (chemiluminescent immunoassay) and estimated folate intake (food frequency questionnaires) were determined. 6-n-propylthiouracil solutions were used to determine bitter taste phenotype (“taster”/”nontaster”). Differences between groups were assessed by t-tests, c2 tests and RR of AP. Results: TAS2R38 haplotype significantly, but only partially predicted taster phenotype (r 1⁄4 0.43, c21⁄435.5, p 50% improvement of symptoms, was assessed. Difference between the groups was tested via Fishers exact test. Results: Of 157 women with IBS, 56 (35%) had a concurrent diagnosis of endometriosis. The median age of those with endometriosis was 32 as opposed to those without where median age was 39 (p 1⁄4 0.014). However, response was reported in 71% with endometriosis compared with 47% of those without endometriosis (p 1⁄4 0.004). Conclusions: The high rate of response to a LFD suggests that this approach should be added to the repertoire of management strategies for endometriosis. Why the response was poorer in those without endometriosis was not clear. Funding source(s): N/A. POSTPRANDIAL NUTRIGENOMIC PATHWAY ANALYSIS OF ADIPOSE TISSUE IN MEN WITH METABOLIC SYNDROME (METS) A.L. Dordevic , M.P. Bonham , A.E. Larsen , P. Gran , A.J. Sinclair , J.B. Jowett , N. Konstantopoulos , D. Cameron-Smith . 1 School of Exercise & Nutritional Science, Deakin University, Australia; Department Nutrition & Dietetics, Monash University, Australia; Metbolic Research Unit, Deakin University, Australia; Genomic & Systems Biology, Baker IDI, VIC, Australia; 5 Liggins Institute, Auckland University, NZ, New Zealand E-mail: aimee.dordevic@monash.edu (A.L. Dordevic) Background/Aims: Profiling the transcriptomic regulation of adipose tissue (AT) by dietary manipulation in MetS will elucidate therapeutic and nutritional targets for chronic disease management. Methods: Seventeen men (8 control; 9 MetS) were recruited to participate in a controlled randomised, single-blinded crossover single meal study. Participants consumed either a saturated fatty acid (FA)-rich breakfast or an unsaturated FA-rich breakfast. AT biopsies were taken at baseline (0 h), and 4 h following each test meal. Global gene expression profiling of the AT was performed using Illumina HumanWG-6 v3 microarray chips followed by gene set enrichment analysis (GSEA) using DAVID software. Pathways were considered significant with Expression Analysis Systematic Explorer (EASE) score < 0.05 and a false discovery rate (FDR) value < 0.05. Results: Two pathways that were differentially regulated in MetS compared to control AT at baseline were also enriched by the following meal consumption. The Lysosome pathway was up-regulated (p < 0.001) and the Ribosome pathway was down-regulated (p < 0.001) in MetS AT compared with controls at baseline. Control AT exhibited enrichment of the Lysosome pathway with the SFA meal (p1⁄4 0.001) and MetS AT showed enrichment of the Ribosome pathway after the UFA meal (p 1⁄4 0.02). Conclusions: Lysosome and Ribosome pathways are impaired in MetS AT and can be manipulated by alterations in meal composition. This finding is important in the discovery of potential nutritional targets for chronic metabolic disease. Funding source(s): Dairy Health & Nutrition Consortium (DHNC). REDUCED FODMAPS IN GLUTEN-FREE GRAINS MAY EXPLAIN THE IMPROVED SYMPTOMS IN PEOPLE WITH IBS FOLLOWING A GLUTENFREE DIET J.G. Muir , J. Mills , D. Suter , F. Bekes , K. Liels , C.K. Yao , P.R. Gibson . Department of Gastroenterology, Central Clinical School, Monash University, Australia; George Weston Foods Ltd, North Ryde, NSW, Australia E-mail: Jane.Muir@monash.edu (J.G. Muir) Background/Aims: Individuals with IBS blame gluten-containing grains for triggering gastrointestinal symptoms. Another component of grains that requires consideration is the FODMAPs mostly fructans and galacto-oligsaccharides (GOS). We aim to analyse the gluten and FODMAP content of 22