A novel antibacterial tripeptide from Chinese leek seeds

Abstract

Antibacterial peptides have been one of the most prospects as a new member of antibiotics in food and/or medicine industry. In this thesis, a novel antibacterial peptide, designated as CLP-1, was purified from Chinese leek seeds by a gel filtration chromatography and reversed-phase high-performance liquid chromatography. CLP-1 was composed of three amino acids, and the sequence was identified as Ser-Asn-Ala (SNA) using LC–MS/MS, which was a novel short antibacterial peptide from Chinese leek seeds. SNA showed moderate antibacterial activity against the growth of Gram-positive and Gram-negative bacteria, and the minimal inhibition concentration values of SNA against Escherichia coli, Staphylococcus aureus, Salmonella, and Bacillus subtilis were 4.31, 2.24, 4.31, and 2.24 mM, respectively. Moreover, SNA exerted slighter hemolytic activity at concentration of 3.44 mM.