Abstract
In this study, a fluorescence strategy based on MPA-AgInZnS quantum dots (AIZS QDs) for the sensitive, effective detection of protamine and trypsin was developed. Protamine as a cationic peptide exhibited electrostatic interactions with AIZS QDs, leading to the aggregation of QDs and subsequent enhancement of the fluorescence intensity. The added trypsin catalyzed the hydrolysis of protamine and led to less formation of AIZS QDs/protamine complex, thereby decreasing the fluorescence intensity. Thus, a method for direct determination of protamine and indirect measurement of trypsin using protamine as a tool was developed. The protamine and trypsin concentrations were determined by the detection of the change in fluorescence intensity. Under the optimized conditions, the fluorescence intensity exhibited a good linear response to the protamine concentration, with a limit of detection (LOD) of 0.13 μg mL−1, and trypsin exhibited a linear relationship, with an LOD of 0.04 μg mL−1. The probe exhibited good biocompatibility and low cytotoxicity according to the MTT assay. Moreover, the application of this probe for intracellular imaging and lysosome localization demonstrates potential development in the biomedical and clinical fields.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.