Abstract
Gold nanoparticles (AuNP) have catalysis on the reaction of HAuCl4-H2O2. The produced AuNP have strong resonance Rayleigh scattering (RRS) effect and surface-enhanced resonance Raman scattering (SERS) effect when Victoria blue B (VBB) and rhodamine S (RhS) were used as probes. The increased RRS/SERS intensity respond linearly with the concentration of gold nanoparticles (AuNPB) which synthesized by NaBH4 over 0.038–76 ng/mL, 19–285 ng/mL, 3.8–456 ng/mL respectively. Four kinds of tested nanoparticles have catalysis on the HAuCl4-H2O2 particles reaction. Thus, a novel nanocatalysis surface plasmon resonance-scattering (SPR-S) analytical platform was developed for AuNP. The DNAzyme strand hybridized with the substrate strand to form double-stranded DNA (dsDNA) which couldn’t protect AuNPc to aggregate to AuNPc aggregations, having strong RRS effect. Upon addition of Pb2+, dsDNA could be cracked by Pb2+ to produce single-stranded DNA (ssDNA) that adsorbed on the AuNPc surface to form AuNPc-ssDNA conjugates. The conjugates have strong catalysis on HAuCl4-H2O2 reaction. With increased Pb2+ concentration, the concentration of AuNPc-ssDNA increased and lead to the catalytic activity stronger. The increased RRS intensity responds linearly with Pb2+ concentration over 16.7–666.7 nmol/L. The SERS intensity responded linearly with the concentration of Pb2+ over 50–500 nmol/L.
Highlights
The SPR-S techniques included the RRS and SERS, which the former is elastic and the later is inelastic scattering that both were based on the nanoparticle scattering
Based on the double-stranded DNA (dsDNA) cracked by Pb(II) to release a short single-stranded DNA that conjugated gold nanoparticles (AuNPs) to form a stable AuNPs-ssDNA complex, and its nanocatalysis of HAuCl4-vitamin C particle reaction, a sensitive RRS method was developed for detection of Pb(II)[27]
When AuNPc-ssDNA solution was used as catalyst, AuNPc modified by aptamer catalytic activity is stronger than AuNPc solution, with the increase of AuNPc-ssDNA concentration, the RRS peak linear increased at 370 nm (Fig. S9)
Summary
The SPR-S techniques included the RRS and SERS, which the former is elastic and the later is inelastic scattering that both were based on the nanoparticle scattering. Pb4+ was reduced to PbH4 gas by NaBH4 and the gas trapped by Au3+ to form nanogold that exhibited a RRS effect at 286 nm This principle was used to detect Pb2+ as low as 7.0 × 10−8 mol/ L26. Based on the dsDNA cracked by Pb(II) to release a short single-stranded DNA that conjugated gold nanoparticles (AuNPs) to form a stable AuNPs-ssDNA complex, and its nanocatalysis of HAuCl4-vitamin C particle reaction, a sensitive RRS method was developed for detection of Pb(II)[27]. There are no reports about the HAuCl4-H2O2 nanogold catalysis SPR-RS analytical platform being utilized to detect trace Pb2+, combing with the DNA enzymes cracked reaction. We have considered the new nanocatalytic reaction of AuNP-HAuCl4-H2O2, and two new SPR methods were developed for detection of Pb(II), combining the analysis platform with the DNAzyme cracking
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